Fig 1: Cytopathic effect (CPE) observed in infected Vero E6 cells. On the harvest day, the cells displayed >75% (3+) CPE. The CPE observed in Vero E6 cells inoculated with the swabs collected from all cases is reported in Table 1. Cells inoculated with serum/plasma specimens showed no CPE and tested negative for MPXV on the harvest day. All mock non-infected cells tested negative for MPXV, and no CPE was observed during the follow-up. Images were captured before the infection and on the harvest day using the EVOS M5000 Imaging System (AMF5000, Thermo Fisher Scientific, Waltham, MA, USA).